artial Purification of Extracellular Lipase from Soil Fungal Isolate
نویسندگان
چکیده
منابع مشابه
Cloning and Enhanced Expression of an Extracellular Alkaline Protease from a Soil Isolate of Bacillus clausii in Bacillus subtilis
in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...
متن کاملPurification and Zymography of lipase from Aspergillus niger PTCC5010
In this study, Aspergillus niger lipase after extraction of medium culture was precipitated with different percentages of acetone and purified by ion exchange chromatography using SP-sepharose HP and Q-sepharose HP. The process of purification of the anzyme was studied by electrophoresis and the molecular weight was detected and determined by Zymography using overlying containing phenol red and...
متن کاملPurification and characterization of an extracellular poly(L-lactic acid) depolymerase from a soil isolate, Amycolatopsis sp. strain K104-1.
Poly(L-lactic acid) (PLA)-degrading Amycolatopsis sp. strains K104-1 and K104-2 were isolated by screening 300 soil samples for the ability to form clear zones on the PLA-emulsified mineral agar plates. Both of the strains assimilated >90% of emulsified 0.1% (wt/vol) PLA within 8 days under aerobic conditions. A novel PLA depolymerase with a molecular weight of 24,000 was purified to homogeneit...
متن کاملcloning and nucleotide sequence of a lipase gene from a soil isolate
the present study was aimed to isolate and characterize the lipolytic enzyme producing bacteria from soil samples of regions around zayande-rood river of isfahan, iran. soil samples were collected from 15 cm depth of soil surface. based on morphology, distinct colonies were isolated and purified through streak culture on to standard agar plates. isolated colonies were examined for lipase activi...
متن کاملCloning, Expression, and Purification of a GDSL-like Lipase/Acylhydrolase from a Native Lipase-Producing Bacterium, Lactobacillus fermentum
Background: Lipase enzymes are of great importance in various industries. Currently, extensive efforts have been focused on exploring new lipase producer microorganism as well as genetic and protein engineering of available lipases to improve their functional features. Methods: For screening lipase-producing lactobacilli, isolated strains were inoculated onto tributyrin agar plates. Molecular ...
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ژورنال
عنوان ژورنال: British Journal of Medical and Health Research
سال: 2018
ISSN: 2394-2967
DOI: 10.46624/bjmhr.2018.v5.i05.001